Iran's Revolutionary Guards warned Saturday that safeguarding security was a "red line" and the military vowed to protect public property, as the clerical establishment stepped up efforts to quell the most widespread protests in years.

The statements came after President Donald Trump issued a new warning to Iran's leaders on Friday, and after Secretary of State Marco Rubio on Saturday declared: "The United States supports the brave people of Iran."

Unrest continued overnight. State media said a municipal building was set on fire in Karaj, west of Tehran, and blamed "rioters." State TV broadcast footage of funerals of members of the security forces it said were killed in protests in the cities of Shiraz, Qom and Hamedan.

Protests have spread across much of Iran over the last two weeks, beginning in response to soaring inflation, but quickly turned political with protesters demanding an end to clerical rule. Authorities accuse the U.S. and Israel of fomenting "the riots." Rights groups have documented dozens of deaths of protesters.

A witness in western Iran reached by phone said the Revolutionary Guards (IRGC) were deployed and opening fire in the area from which they were speaking, declining to be identified for their safety.

In a statement broadcast by state TV, ⁠the IRGC -- an elite force which has suppressed previous bouts of unrest -- accused terrorists of targeting military and law enforcement bases over the past two nights, killing several citizens and security personnel and saying property had been set on fire.

Safeguarding the achievements of the 1979 Islamic revolution and maintaining security was "a red line," it added, saying the continuation of the situation was unacceptable.

The military, which operates separately ​from the IRGC but is also commanded by Supreme Leader Ayatollah Ali Khamenei, announced it would "protect and safeguard national interests, the country's strategic infrastructure, and public property."

In a country with a history of fragmented opposition to clerical rule, the son of the last shah of Iran who was toppled in the 1979 Islamic revolution has emerged as a prominent voice abroad spurring on the protests.

In his latest appeal on the X social media platform, U.S.-based Reza Pahlavi said: "Our goal is no longer merely to come into the streets; the goal is to prepare to seize city centers and hold them."

He also called on "workers and employees in key sectors of the economy, especially transportation, and oil, and ⁠gas and energy," to begin a nationwide strike.

Trump said Thursday he was not inclined to meet Pahlavi, a sign that he was waiting to see how the crisis plays out before backing an opposition leader.

Trump, who bombed Iran last summer and warned Tehran last week the U.S. could come to the protesters' aid, issued another warning on Friday, saying: "You better not start shooting because we'll start shooting too."

"I just hope the protesters in Iran are going to be safe, because that's a very dangerous place right now," he added.

Some protesters on the streets have shouted slogans in support of Pahlavi, such as "Long live the shah," although most chants have called for an end to rule by ⁠the clerics or demanded action to fix an economy hammered by years of U.S. and other international ​sanctions and pummeled by the 12-day war in June, when Israel and ⁠the U.S. launched airstrikes on Iran.

A doctor in northwestern Iran said that since Friday, large numbers of injured protesters had been brought to hospitals. Some were badly beaten, suffering head injuries and broken legs and arms, as well as deep cuts.

At least 20 people in one hospital had been shot with live ammunition, five of whom later died.

On Friday, Khamenei accused protesters of acting on behalf of Trump, saying rioters were attacking public property and warning that Tehran would not tolerate people acting as "mercenaries for foreigners."

The Revolutionary Guards' public relations office said three members of ‌the Basij security force were killed and five wounded during clashes with what it described as "armed rioters" in Gachsaran, in the southwest.

Another security officer was stabbed to death in Hamedan, in western Iran. The son of a senior officer, Brigadier General Martyr Nourali Shoushtari, was killed in the Ahmadabad area of Mashhad, in the northeast. Two other security personnel were killed over the past two nights in Shushtar, in Khuzestan province.

The protests pose the biggest internal challenge in at least three years ​to Iran's clerical rulers, who look more vulnerable than during past bouts of unrest amid a dire economic situation and after last year's war.

The leaders of France, Britain and Germany issued a joint statement Friday condemning the killing of protesters and urged the Iranian authorities to refrain from violence.

Authorities have described protests over the economy as legitimate while condemning what they call violent rioters and cracking down with security forces.

Iran's clerical establishment has weathered repeated past bouts of unrest, including student protests in 1999, over a disputed election in 2009, against economic hardships in 2019, and the 2022 Woman, Life, Freedom protests.

Iranian rights group HRANA said it had documented 65 deaths including 50 protesters and 15 security personnel as of Jan. 9. The Norway-based human rights group Hengaw said more than 2,500 people had been arrested over the past two weeks.